Speciality Immunoassays and Reagents

Cell Proliferation kit with XTT Reagent

Catalog Number 409005

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Cell proliferation assays are widely used in cell biology for the study of growth factors, cytokines and media components, for the screening of cytotoxic agents and for lymphocyte activation. The need for a reliable, sensitive and quantitative assay that would enable analysis of a large number of samples led to the development of methods, such as:

• use of radioactive thymidine to label DNA in live cells
• use of Brdu to label DNA in live cells (as a substitute for radioactive thymidine)

The advantages of using this kit can be summarized with the following attributes:

• easy-to-use: there is no requirement for additional reagents and/or the cell washing procedures
• speed: multiwell plates and an ELISA reader can be used for reading
• sensitivity: can be assayed even in low cell concentrations
• accuracy: dye absorbance is proportional to the number of cells in each well
• safety: there is no need for radioactive isotopes

 

 

Kit Components

XTT Reagent (10x5ml)

A sterile solution containing the XTT reagent. The solution should be stored frozen and should not be exposed to light. To avoid repeated re-freezing, dividing the solution into a number of vials after defrosting the original vial is recommended.

Note: if sediment is present in the solution, heat the solution to 37°C and swirl gently until a clear solution is obtained.

 

Activation Reagent (2x0.5ml)

A sterile solution containing PMS (N-methyl dibenzopyrazine methyl sulfate). The solution should be stored frozen and should not be exposed to light. To avoid repeated re-freezing, dividing the solution into a number of vials after defrosting the original vial is recommended.

 

Product inserts are for information use only.

MDB 409005 MSDS