IHC staining using Tyrosine hydroxylase antibody - used for staining of Dopaminergic neurons, as TH is one of the enzymes involved in the production of Dopamine in neuronal cells. This staining is used in MPTP-induced PD model in mice or 6-OHDA-induced PD model in rats.
Brain sections of 6-OHDA induced Parkinson’s disease model in rats
Figures A and B shows subgross image (A) and high magnification (B) of Sunstantia Nigra sections showing reduction in number of TH-positive cells in the right hemisphere vs. the left intact hemisphere. Concomitantly, Figure C is subgross image of TH-positive nerve fibers in Striatum section of the Left intact hemisphere in comparison to the lack of staining in the 6-OHDA injected Right hemisphere. See images below:
6-OHDA induced Parkinson’s disease