Collagen-induced arthritis (CIA)

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Collagen-Induced Arthritis (CIA): Pre-clinical efficacy models

The Collagen Induced Arthritis (CIA) model is a commonly used model as it shares immunological and pathological similarities to human RA. Arthritis is initiated by intradermal injections of Collagen Type II (CII) emulsified in Complete Freund's Adjuvant (CFA). This causes an immune response generating antibodies to CII. Therefore there is both a T cell and B cell component to the pathology. The joint destruction involves cartilage destruction, bone resporption, synovial hyperplasia and periarticular cell infiltration thereby sharing many similarities to human RA. The mCollagen Induced Arthritis model contains more neutrophils and less monocytes/macrophages than the human disease. rCollagen-Induced Arthritis has a quicker onset of disease and shorter duration.

 

mCollagen Induced Arthritis:

Length 42 days
Strain DBA
Positive Controls Dexamethasone, Indomethasone, Methotrexate or Enbrel
Induction Various depending on requirements (severe disease, mild disease etc).
Measurements Body weights, Clinical signs, Arthritis score
Optional Readouts Hind paw thickness, Histology, Cytokine analysis, Gene expressio

 

rCollagen-Induced Arthritis

Length 35 days
Strain Lewis
Positive Controls Dexamethasone
Measurements Body weights, Clinical signs, Arthritis score, Ankle joint thickness, paw volume
Optional Readouts Histology, Cytokine analysis, Gene expression