Speciality Immunoassays and Reagents

PCR Mycoplasma Test Kit

Catalog Number 409010

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EZ-PCR Mycoplasma Test Kit is designed to detect the presence of mycoplasma contaminating biological materials, such as cultured cells. Mycoplasma detection by the direct culture procedure is time-consuming and some mycoplasma species are difficult to cultivate. With PCR testing, results are obtained within a few hours, since the presence of contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis. There is no need to prepare probes labeled with radioisotopes, or to calculate enzyme, dNTP’s or buffer concentrations. Instead, a ready-to-use, optimized PCR mix is supplied. The primer set allows detection of various mycoplasma species (M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum and M. capricolum), as well as Acholeplasma and Spiroplasma species, with high sensitivity and specificity.

 

Kit Components:

1. Reaction Mix 200µl
2. Buffer Solution 1ml
3. Positive Template Control 20µl

Reagents not supplied in the kit:

1. Mineral Oil
2. Agarose gel
3. Distilled Sterilized water

Equipment required:

1. Authorized thermal cycler for PCR
2. Microcentrifuge tubes
3. Agarose gel electrophoresis apparatus
4. MicrocentrifugeMicropipets and pipette tips (autoclaved)

 

Product inserts are for information use only.

MDB 409010 MSDS