Speciality Immunoassays and Reagents

Rat Prolactin ELISA

Rat Prolactin ELISA				Catalog # RPRL.96
Format	Competitive assay				View product insert
Size/Volume	96 wells
Species	rat				Request Quote
Sample Type	serum, plasma
Sample Preparation	-
Sample Size	50 uL
Standard Curve Range	0.39 - 50 ng/mL
Sensitivity	0.2 ng/mL
Assay Length	overnight incubation

 

OVERVIEW

Intended use

The Rat Prolactin ELISA kit is intended for the quantitative determination of prolactin in rat serum plasma. This assay is intended for in vitro research use only.

Principle of the assay

This Enzyme Immunoassay (EIA) is based on the competition between unlabelled rat prolactin and acetylcholinesterase (AChE) linked to rat prolactin (tracer) for limited specific rabbit anti-rat prolactin antiserum sites.

The complex rabbit antiserum-rat prolactin (free prolactin or tracer) binds to the mouse monoclonal anti-rabbit antibody that is attached to the well.

The plate is then washed and Ellman's Reagent (enzymatic substrate for AChE and chromogen) is added to the wells.

The AChE tracer acts on the Ellman's Reagent to form a yellow compound.

The intensity of the colour, which is determined by spectrophotometry, is proportional to the amount of tracer bound to the well and is inversely proportional to the amount of free rat prolactin present in the well during the immunological incubation.

Specification

•     Cross-reactivity with rat LH, GH and TSH: <0.01%

•     Assay range 0.39-50 ng/mL

 

Product inserts are for information use only.