Collagen Induced Arthritis: Reagents and Assays

Rapid Collagenase Assay Kit

Recombinant Human MMP-13

OVERVIEW
View product insert
Request Quote

Rapid Collagenase Assay Kit
This kit contains all the necessary reagents to assay for collagenase activity including recombinant, human, neutrophil procollagenase as a standard and two types of collagenase activators. Unlike antibody-based ELISA kits that only detect the presence of enzymes, this kit is designed to assay for mammalian collagenase activity (see references 7-9). The assay uses FITC-labelled soluble bovine type I collagen as a substrate and only requires two hours.

The rapid assay method consists of four steps:

  1. Activation of latent collagenase in test samples,
  2. Reaction with substrate for 10-60 minutes,
  3. Denaturation and extraction of degraded products, and
  4. Measurement of fluorescence intensity (FI) in the extract.
The enzyme reaction is 10 times faster than substrate gel analysis and much more sensitive than assays using radiolabeled collagen.

Kit includes labelled collagen to perform 100 assays.

 

Catalog No. Contents Suitable for
Substrate Standard
CGKIT-I
FITC-Bovine
Type I Collagen
(10 mg, for 100 assays)
Recombinant Human
MMP-8 (latent form), 1 Unit
All Collagenases
(100 assays)
CGKIT-II
FITC-Bovine
Type II Collagen
(10 mg, for 100 assays)
Recombinant Human
MMP-8 (latent form), 1 Unit
All Collagenases
(100 assays)

 

Product inserts are for information use only.