Cell Culture Related Kits

Cell Proliferation Kit

Product Description	Size	Catalog #
Cell Proliferation Kit with XTT Reagent	1000 assays	409005

Cell proliferation assays are widely used in cell biology for the study of growth factors, cytokines and media components, for the screening of cytotoxic agents and for lymphocyte activation. The need for a reliable, sensitive and quantitative assay that would enable analysis of a large number of samples led to the development of methods, such as:

• use of radioactive thymidine to label DNA in live cells
• use of Brdu to label DNA in live cells (as a substitute for radioactive thymidine)

The advantages of using this kit can be summarized with the following attributes:

• easy-to-use: there is no requirement for additional reagents and/or the cell washing procedures
• speed: multiwell plates and an ELISA reader can be used for reading
• sensitivity: can be assayed even in low cell concentrations
• accuracy: dye absorbance is proportional to the number of cells in each well
• safety: there is no need for radioactive isotopes

 

PCR Mycoplasma Test Kit

Product Description	Size	Catalog #
PCR Mycoplasma Test Kit	20 assays	409010

EZ-PCR Mycoplasma Test Kit is designed to detect the presence of mycoplasma contaminating biological materials, such as cultured cells. Mycoplasma detection by the direct culture procedure is time-consuming and some mycoplasma species are difficult to cultivate. With PCR testing, results are obtained within a few hours, since the presence of contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis. There is no need to prepare probes labeled with radioisotopes, or to calculate enzyme, dNTP’s or buffer concentrations. Instead, a ready-to-use, optimized PCR mix is supplied. The primer set allows detection of various mycoplasma species (M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum and M. capricolum), as well as Acholeplasma and Spiroplasma species, with high sensitivity and specificity.

 

---

 

 

 

Product inserts are for information use only.