Inflammatory Assays

 

Collagen Type II ELISA

Collagen Type II ELISA				Catalog # CII96
Format	Sandwich ELISA				View product insert
Size/Volume	96 well
Species	human, mouse, rat, porcine, chick				Request Quote
Sample Type	cell culture supernate
Sample Preparation	collagen must be solubilized
Sample Size	100 uL
Standard Curve Range	4.7 - 300 ng/mL
Sensitivity	< 2.0 ng/mL
Assay Length	<5 hours

Intended use

The Collagen Type II ELISA provides a method for the determination of collagen type II (CII) in human, mouse, rat, porcine, and chick cell culture supernate samples.

Principle of the test

ELISA or enzyme-linked immunosorbent assay is a colorimetric based immunoassay utilizing a capture antibody and a detection antibody to provide a unique and powerful assay system. The Collagen Type II (CII) ELISA is a hetergoenous sandwich ELISA where antibody to collagen type II is pre-coated onto a 96-well microplate and any collagen type II present in the sample will be bound onto the  microplate by the antibody. A secondary biotinylated antibody is then added and converted by the enzyme to produce a quantifiable colored product that is in direct proportion to the amount of antigen bound in the initial reaction.

 

Product inserts are for information use only.