Cytokine-stimulated IL-6 and IL-8 production from keratinocytes is an effective early screening tool for anti-inflammatory compounds.
Keratinocytes, the major cell type of the epidermis, are key modulators of inflammation in the skin. In the cytokine-stimulated keratinocyte assay, an inflammed state is mimicked by treating normal human epidermal keratinocytes with pro-inflammatory (IL-1a and/or TNF-a). After treatment, cell culture supernatants are assayed for inflammatory mediator production using a bead-based multiplex assay system.
| Cytokine-stimulated kertinocyte assay Get Proposal |
| Cell System |
Pooled normal neonatal humen epidermal keratinocytes (NHEK)
|
| Length |
1 week |
| Stimulant |
Cytokines (IL-1a and/or TNF-a)
|
| Reference |
Dexamethasone |
| Readouts |
IL-6 and IL-8 (additional analytes available on request)
|
Figures: NHEKs were stimulated with IL-1b and/or TNF-a. Following treatment with vehicle or dexamethasone, cell culture supernatants were assayed for IL-6 and IL-8 using a bead-based multiplex assay. Mean values are shown. Error bars represent standard deviations.