T cell activation assay: preclinical research

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Histamine-stimulated calcium flux in vitro assay

Human bronchial smooth muscle cells are loaded with a calcium indicator dye and exposed to histamine. The calcium response is monitored by following the increase in fluorescent intensity over time.

In vitro histamine-stimulated calcium flux assay    Get Proposal
Cell System Human bronchial smooth muscle cells(BSMC)
Length 1 week
Stimulant histamine
Reference inquire
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Calcium indicator dye (fluorescence)

 

histamine-stimulated calcium flux in vitro assay

Figure: Internal calcium store release: maximum region 2 - maximum region 1. Extracellular calcium influx: maximum region 4 - minimum region 3.


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