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Preclinical Laboratory Services

Bleomycin-Induced Fibrosis

Validated Model of Idiopathic Pulmonary Fibrosis/Lung Injury

 

Bleomycin-induced Lung Injury/Pulmonary Fibrosis (IPF)

Bleomycin is a chemotherapeutic agent used to treat cancers such as Hodgkins lymphoma. One of the side effects is pulmonary toxicity, which can be life threatening in approximately 10% of patients.  

 The mechanism of bleomycin-induced lung injury includes oxidative damage via oxidant-mediated DNA breaks, causing inflammatory reactions in the lungs.

Bleomycin has also been used to induce lung injury in rodents for basic research into pulmonary fibrosis for over a decade. At MD Biosciences, we have refined the oral aspiration administration to allow for more even distribution of disease throughout both right and left lungs, making bleomycin-induced injury in mice a reliable model for research. This is a great model for studying idiopathic pulmonary fibrosis (IPF).
 

Available Bleomycin Study Readouts:

  • Immunophenotyping of bronchoalveolar lavage fluid (BALF):
    •   Quantitation of T Cells, B Cells, Macrophage, Neutrophils, Eosinophils, Fibroblasts, etc
  • Cytokine Expression Levels (ELISA/Multiplex) in:
    • BALF
    • Lung Tissue Homogenate
    • Plasma/Serum
  • Histology of lung:
    • H&E for morphologic analysis, cellular infiltration, etc (Figure 1)
    • Masson's Trichrome staining for collagen (Figure 2)
    • IHC for specific markers of interest such as CD3, CD11b, etc (Figures 3 & 4)
  • Lung tissue biomarkers
  • Blood biomarkers (protein or nucleic acid)
  • Lung tissue cellularity 

Figure 1. Late Stage Histology from Control & Bleomycin-treated Mouse Lung

Public_Bleo_Histology_Fig

Figure 2. Lung Sections from Belomycin-Induced Fibrosis Model Stained For Macorphage (IHC Staining with CD11b Antibody):

Lung-Sections-Bleomycin-Fibrosis-IHC-Staining-CD11b-Macrophage-Marker.png

A. Medium magnification of interstitial perivascular infiltrates showing staining for CD11b-positive cells (macrophage-specific marker). B. High magnification of interstitial perivascular infiltrates of CD11b-positive cells.

 

Figure 3. Lung Sections From Bleomycin-Induced Fibrosis Model Stained for Lymphocytes (IHC Staining with CD3 Antibody):

Lung-Sections-Bleomycin-Induced-Fibrosis-IHC-Staining-CD3.png

A. Rare CD3+ cells (arrows, non-specific staining in epithelium in upper left corner of image). Figure B. Subpleural infiltrate which includes CD3+ lymphocytes 

Species Availability:

Mouse

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