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Preclinical Laboratory Services

TNBS-Induced Model of IBD

Model of Gastrointestinal Inflammation and Crohn's Disease

TNBS-induced intestinal inflammation is commonly used in mice as a model for IBD, particularly in reference to Crohns disease (CD).
 
IBD Model Utilizes Intrarectal Administration of TNBS 
For our 7-day TNBS model, we induce disease through intrarectal administration of TNBS, to produce clinically amenable indicators to that of Crohn's Disease. Indicators include: diarrhea, rectal bleeding, body weight loss, more diffuse intestinal inflammation, occasional adhesions, fibrosis and vascularized ulceration. Disease indications can also be significantly reduced for a less servere phenotype via a one-time i.p. injection of anti-p40 antibody. 
 
Our model is available in both Balb/c mice and rats, and is pathologically driven by Th1/Th17 dependant mechanisms.

 

Speak to a scientist about your study requirements or any options not listed below today!

Dosing Routes: Assessments available:
PO Body weight loss
IV Colitis score
IP Colon length/weight
SC Gross Pathology
Infusion Pump Biomarkers
  Histology/IHC
  PK

 

Colonic Sections from TNBS-Induced IBD Model in Mice Processed via "Swiss Roll" Technique to Preserve Morphology (Periodic Acid Schiff/PAS Staining):

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Figure A. Medium magnification of PAS-stained colon sections demonstrating mucins and polysaccharides. Figure B. High magnification of PAS-stained section in A.

 

Colitis Scores in the TNBS Model 

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Balb/c were administered TNBS intra-rectally. Over the course of 7 days, colitis score was monitored in animals receiving vehicle and positive controls anti-IL-12 p40. 

 SPECIES AVAILABLE:

  • mouse TNBS
  • rat TNBS
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