MD Biosciences performs efficacy studies for proof-of-concept as well as assays for early screening and profiling of drug candidates. If there is a model or assay that you don't see listed, please contact a representative to discuss a custom assay design.
TNBS-induced IBD
The hapten induced model of colitis induced by intrarectal administration of colitis resembling Crohn's Disease in the distribution and location of lesions and allows the study of the early events in the initation of colitis. The pathology is driven by a potential initial IL-12 and TNF-a response, which preclude a counter regulatory IL-10 and TGF-b response.
DSS-induced IBD
This model employs dextran sodium sulpahte to induce colitis and runs for 7 days. In contract to TNBS-induced colitis, the pathology is not dependant on T cells and is therefore a more useful model to study the role of non lymphoid cells such as macrophages in induction of disease and is similar to ulcerative colitis.
Adenocarcinoma Cell Based Assay
An early event thought to participate in the pathogenesis of IBD is the disruption of the gastrointestinal epithelial barrier. This disruption leads to the mixing of microbial pathogens from lmen with antigen presenting cells in the lamina propria producing an inflammatory response. The resulting pro-inflammatory cytokines and chemokines recruit and activate leukocytes, regulate the integrity of the epithelial barrier and stimulate the production of chemokines from epithelial cells. Together these events lead to the inflammation of the intestines.
The human adenocarcinoma cell line HT29 is stimulated with TNF-a to mimic chronic inflammation.
Intestinal Cell Damage Assay
A monolayer of human colon adenocarcinoma cells is cultured on a semipermeable support membrane above LPS-stimulated macrophages. Macrophage-induced epithelial monolayer damage is determined by measuring the transepithelial electrical resistance (TEER).
Inflammatory Bowel Disease (IBD)
IBD is a common chronic gastrointestinal disorder whose incidence occurs in more than 100,000 cases per year depending on the geographical location. IBD is an umbrella term that covers a range of diseases including ulcerative colitis (UC) and Crohn's Disease, both which are chronic wasting conditions of the unknown aetiology for which there is still no cure. They are characterized clinically by diarrhea and body weight loss. Histopathological signs include leukocyte infiltration, pronounced epithelial hyperplasia, depletion of mucin secreting goblet cells and finally ulceration.