T cell activation assay: preclinical research

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iNOS Induction Assay: Preclinical Contract Research

 

Nitric Oxide (NO) is an anti-bacterial and anti-viral effector of the innate immune system produced by activated immune cells such as monocytes, macrophages, neutrophils and natural killer (NK) cells. Microbial cell wall components and cytokines released from host cells can stimulate the production of inducible NO synthase (iNOS), which in turn generates NO.

The iNOS induction assay can be used to screen for compounds that induce or inhibit iNOS production in mouse macrophages. 

 

iNOS Induction Assay                     Get Proposal
Cell System Mouse Macrophage Cells (RAW 263.7)
Length 1 week
Stimulant LPS and IFN-g (for inhibition screen)
Reference LPS and IFN-g(for induction screen)
Readouts

Total NO (Griess reaction)

 

iNOS induction assay - in vitro screening - MD Biosciences preclinical contract research

Figure: iNOS induction assay. RAW 263.7 cells were treated with vehicle or LPS and IFN-g. After treatment, cell culture supernatants were assayed for total nitric oxide. Mean values are shown. Error bars represent standard deviations. 

 

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