The focal cerebral ischemic model of stroke is commonly used to assess ischemic injury in the brain. Occlusion of the middle cerebral artery (MCA) leads to a reduction of cerebral blood flow in both the striatum and the cortex, but the degree largely depends on the duration and site of occlusion. It is a highly reproducible model that results in focal brain damages similar to those occurring in human stroke. One can choose a permanent occlusion which produces massive damage or transient occlusion where the artery is occluded for a period of time before reperfusion.
The MCAo model can be used to evaluate stroke or neurodegeneration as a result of oxygen stress.
Ischemic MCAo models for stroke:
|Expected Area of Damage:||Cortex Stratum/Globus Plidus, Thalamic Nucleus, Hoppocampus||Mainly Cortex|
|Cognitive/Behavior Tests:||Spatial memory, contralateral motor function and coordination (Staircase, Adhesion removal test, water maze, grip test)||Contralateral motor function and coordination (Staircase, adhesion removal test, grip test)|
|Histology Assessment:||Thionin/H&E for infarct size & assessment||Thionin/H&E for infarct size & assessment|
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Coronal Sections of Rat Brain Tissue Demonstrating Infacrt Size Assesment (Thionin Staining):
Figures A & C. Normal Rat Brain Tissue. Figures B. & D. Rat brain 72 housr post-MCAo demonstrating clear damage due to infarct in the Right hemisphere.