Inflammatory Assays

 
Mouse IgG2b Anti-Collagen Type II ELISA

Mouse IgG2b Anti-Collagen Type II ELISA Specifications Cat # CII2B96
Format Sandwich ELISA
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Size/Volume 96 well
Species mouse
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Sample Type serum
Sample Preparation dilute samples at least 1:100
Sample Size 100 uL
Standard Curve Range 0.025 - 2.0 ng/mL
Sensitivity < 0.02 ng/mL
Assay Length <2.75 hours

This assay is designed to measure mouse IgG2b collagen type II antibodies generated specifically against the species of collagen type II used to induced arthritis. Please select the catalog # that corresponds to the collagen used for arthritis induction.

Collagen Species used for induction Sample Catalog #
Bovine CII Mouse serum CII2B96-B
Chicken CII Mouse serum CII2B96-C
Mouse CII Mouse serum CII2B96-M
Porcine CII Mouse serum CII2B96-P
Rat CII Mouse serum CII2B96-R
Human CII Mouse serum CII2B96-H

Intended use

ELISA or enzyme-linked immunosorbent assay is a colorimetric based immunoassay utilizing a capture antibody and a detection antibody to provide a unique and powerful assay system. Collagen Type II is widely used for inducing disease in the collagen-induced arthritis (CIA) models. The serum antibodies generated are highly specific to the type II collagen used for immunization. This assay is designed to measure type II collagen antibodies in mouse serum when bovine collagen type II is used for immunization.

 

Product inserts are for information use only.