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Neurology Disease Models

Multiple Sclerosis in-vivo model

Multiple Sclerosis

Multiple sclerosis is a demyelinating disease of the CNS. Although no animal model thus far establish all facets of the human MS, EAE induced in rodents represents the disease model most studied for the disease. Multiple sclerosis is one of the most common disabling neurological diseases in young adults. The main features of the disease are focal areas of demyelination and inflammation. The pathogenesis of the disease is unclear. The disease course is unpredictable and life-long. The disease affects women more commonly for unknown reasons. The etiology of the disease seems to be dependent on genetic and environmental factors. Several studies have suggested viral infections as a contributor to the disease. EAE is mediated by autoimmune CD4+ T-Cells. These cells develop in the peripheral lymphoid organs and travel to the CNS causing an autoimmune response. The development of T cells is controlled largely by the expression of various cytokines as well as cellular adhesion molecules. The origin of the model is traced to the development of the rabies vaccine. Encephalomyopathy was caused in a small percentage of humans who received the rabies vaccine. Subsequent studies succeeded in inducing the paralytic disease in different animals including rabbits. Finally methods were developed to cause inflammatory reaction as well as demyelination with limited number of injections. The EAE model today is the most thoroughly studied animal model for human autoimmune diseases.

MD Biosciences Multiple Sclerosis In-vivo Models

 Type Model  Description
 In Vivo MOG-induced EAE  

Myelin oligodendrocyte glycoprotein (MOG) is used to initate disease.

  
       

 In Vivo

 PLP-induced EAE  

Sections of proteolipid protein (PLP-139-151), which is only found in myelin, is used to initate disease induction along with injections of pertussis toxin. Model can be stopped at 21 days or continued for re-lapse.

   
       

 In Vivo

 MBP-induced EAE   MBP, a major consituent of myelin, is used to initate disease induction. Model is self limiting and is 14-20 days.
  
       

Custom designed models and in vitro assays available. Please contact a representative to discuss a custom assay design.