Preclinical Contract Research

Neuroinflammation

LPS-induced Neuroinflammation

LPS-induced Neuroinflammation

Inflammation 1.jpg
 
In the CNS, glia cells provide support to neural function and respond to stress and insults by upregulating inflammatory processes. Under normal conditions, these responses are kept in-check by anti-inflamamtory and neuroprotective mechanisms. However, in a diseased brain the dysregulation of glial cells promotes neuroinflammation that can lead to degeneration of neurons. Astrocytes react to injury by hypertrophy and upregulation of the glial-fibrillary acidic protein. 
 
LPS-induced Acute Neuroinflammation Model
Our LPS-induced acute neuroinflammation model provides a quick assessment of the number of activated microglia and astrocytes in the hippocampus. Following induction with LPS, brains are removed for histological assessment of the neuroimmunoreactive cells using the following markers:  
  • F4/80 for microglia cells (see graph below)
  • Glial-Fibrillary Acidic Protein (GFAP) IHC for astrocyte demonstration

 Brain section from LPS-Induced Neuroinflamation Model in Mice (IHC Staining with Anti-GFAP Antibody):

GFAP-IHC-Staining-Brain-Section-LPS-Induced-Neuroinflammation-Model_Mice.png

A.                                                                                                                                  B.

Figure A. Medium magnification and Figure B. High magnification of GFAP-positive astrocytes in brain sections of LPS-induced neuroinflammation.

Our scientists are experienced with rapidly validating this type of model. Contact a scientist to discuss your neuroinflammation study requirements today. 

 

SPECIES AVAILABLE:

  • mouse
  • rat

 

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