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Neuroscience Laboratory Services

MBP-Induced EAE

Multiple Sclerosis Model 

Self-remitting myelin basic protein (MBP) induced EAE Efficacy Model for Multiple Sclerosis (MS)

When conducting MS research, it has been found that administration of one dose of MPB in a Lewis rat leads to a single relapse that's characterized mainly by hind paw paralysis. Such a model can be characterized, on the histology level, by infiltration of mononuclear cells to the cervical spinal cord and formation of foci.This model is self-limiting and does not show demyelination.

MBP-induced EAE Efficacy Model at MD Biosciences subjects Lewis rats to an MBP (emulsified with CFA) injection on day 0, where the disease develops between days 10-13, and fully recovers between day 18-21. 

Model Assessments Include:

  • Clinical score
  • Biomarkers
  • Histology for cellular infiltration
  • Immuno-histochemistry (IHC)
  • Pharmacokinetics (PK)

Brain tissue - H&E Staining

MD Biosciences offers H&E staining of brain tissue for detection of inflammatory cells infiltrating into the spinal cord. 

H&E Staining of Brain Tissue from MBP-Induced EAE Model


Figure A. Low magnification and Figure B. High magnification of transverse sections of brain tissue demonstrating severe infiltration of inflammatory cells into the white matter.

H&E Staining of Transverse Section of Spinal Cord from MBP-Induced EAE Model


(A-D, Vehicle Group, Inflamed Spinal Cord Tissue): Figure A. Low magnification of inflammatory infiltrate in the white matter of the spinal cord. Figure B. Medium magnification of foci of inflammatory infiltrate. Figure C. High magnification shows mixed populations of mononuclear cells (black arrows). Figure D. Medium magnification of foci of inflammatory cells (black arrowheads).

E-G (Dexamethasone Group, normal appearance of the spinal cord): Figure E. Low magnification of the spinal cord showing minimal inflammatory infiltrate. Figure F. Medium magnification of a minimal foci of inflammatory infiltratate (circled). Figure G. High magnification of circled area in F showing several mononuclear cells (black arrows) surrounded by 2 blood vessels (asterisks) at the junction of the  gray and white matter (black lines).

Our scientists are highly experienced with MS models. Contact a scientist to discuss your study requirements today!


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preclinical models of EAE for the study of MS (MOG-EAE, MBP-EAE, PLP-EAE)


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