MBP-CFA-Induced EAE in Lewis Rats

Self-remitting MBP-induced EAE Efficacy Model for MS Research

When conducting MS research, it has been found that one dose of MPB in a Lewis rat leads to a single relapse that's characterized mainly by hind paw paralysis. And on the histology level, such a model can be characterized by infiltration of mononuclear cells to the cervical spinal cord and formation of foci. Unfortunately, this model can be self-limiting and does not show demyelination.
 
Self-remitting MBP-induced EAE Efficacy MS Model
 
At MD Biosciences, we offer a robust self-remitting MBP-induced EAE efficacy model for MS research. Our model subjects Lewis rats to an MBP (emulsified with CFA) injection on day 0, where the disease develops between days 10-13, and full disease recovery occurring between
days 18-21. 

Our Model Assessments Include:

  • Clinical Score
  • Biomarkers
  • Histology for cellular infiltration
  • Immunohistochemistry (IHC)
  • PK

Brain tissue - H&E staining

We also offer H&E staining of brain tissue for detection of inflammatory cells infiltrating into the spinal cord.

H&E Staining of Brain Tissue from MBP-Induced EAE Model

MBP-Induced-EAE-H&E-Staining-Brain-Tissue-Spinal-Cord.png 

Figure A. Low magnification and Figure B. High magnification of transverse sections of brain tissue demonstrating severe infiltration of inflammatory cells into the white matter.

 

H&E Staining of Transverse Section of the Spinal Cord from MBP-Induced EAE Model in Rats

MBP-Induced-EAE-Spinal-Cord-Tissue-H&E-Staining.png

(A-D, Vehicle Group, Inflammed Spinal Cord Tissue): Figure A. Low magnification of infammatory infiltrate in the white matter of the spinal cord. Figure B. Medium magnification of foci of inflammatory infiltrate. Figure C. High magnification shows mixed populations of mononuclear cells (black arrows). Figure D. Medium magnification of foci of inflammatory cells (black arrowheads).

E-G (Dexamethasone Group, normal appearance of the spinal cord): Figure E. Low magnification of the spinal cord showing minimal inflammatory infiltrate. Figure F. Medium magnification of a minimal foci of inflammatory infiltratate (circled). Figure G. High magnification of circled area in F showing several mononuclear cells (black arrows) surrounded by 2 blood vessels (asterisks) at the junction of the  gray and white matter (black lines).


Our scientists are highly experienced with MS models. Contact a scientist to discuss your study requirements today!

 

SPECIES AVAILABLE:

  • rat MBP

 

Request Study Proposal
preclinical models of EAE for the study of MS (MOG-EAE, MBP-EAE, PLP-EAE)

CONTACT US:

1-888-876-3246
+41-44 986-2628
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