PLP is a major protein component of CNS myelin. Sections of PLP, such as 139-151 peptide, are encephalitogenic to certain mouse strains. PLP injected in Swiss Jim Lambert (SJL) mice together with pertussis toxin (PT) will lead to remitting- relapsing disease. Relapses are associated with epitope spreading in which T cell reactivity to secondary endogenous peptides that emerge as a consequence of the initial phase of myelin destruction. Remissions are associated with a temporary loss of inflammatory cells from the CNS.
Remitting relapsing PLP-induced EAE Efficacy Model for MS Research
In our Multiple Sclerosis (MS) efficacy model, disease is induced on day 0 using PLP emulsified with CFA. Additionally, to increase the susceptibility of the BBB, all animals are subject to supplemental immunostimulation with a PT injection on day 0 and day 2. The disease typically develops between days 10–12 with the peak occurring between days 14-16. If the relapse phase is required, the study can be extended such that a second disease peak will be apparent between days 25-30. This disease is characterized by paralysis attacks of all 4 paws and is typically used for testing the activity of a compound by injection of the compound between phases.
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