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MD Biosciences Blog

MD Biosciences Develops Large Animal Neuropathic Pain Model

Posted by MD Biosciences on Oct 3, 2012 6:48:00 AM

The porcine sciatica model can evaluate efficacy and PK/PD from the same subject and may provide more clinically relevant data at preclinical stages minimizing expensive failures.

MD Biosciences has developed a large animal model of neuropathic pain for preclinical evaluation of analgesic or neuroprotective therapies. The sciatica model was developed in the pig, as the innervation is similar to that of humans, providing more clinically relevant data at the preclinical stages. The model is suitable for evaluating both efficacy and PK/PD in the same animal and can be used for systemic, local and cell therapies as well as slow release drugs and devices.

Creating injury directly on the sciatic nerve by either nerve crush or nerve cutting induces neuropathic pain and minimizes the inflammatory component. The nerve crush method produces a less pronounced pain as well as a quicker recovery for the subjects. For therapies that are thought to be neuroprotective or promote nerve regeneration, partial nerve damage using the nerve crush method will allow direct treatment to the nerve. Using either method, subjects are completely recovered by 7 days post surgery and can be evaluated for pain up to 21 days. In addition to sensitivity to pain and pain sensation, subjects avoid using the ipsilateral leg suggesting a responsiveness to weight bearing and sustained pain.

 

Readouts:

  • Von Frey
  • Cold allodynia
  • Pin prick
  • Weight bearing
  • Histology

 

Pain threshold in the large animal model of neuropathic pain

Figure: Subjects underwent either nerve crush or nerve cut methods. Subjects were tested for sensitivity to Von Frey pre- and post-treatment with 1 mg/kg morphine. Data is reported using Von Frey method and shows the 50% threshold in grams (g). 

 

The porcine sciatica model of neuropathic pain is just one of many large preclinical models offered by MD Biosciences. With the high failure rate at clinical trial phases, more relevant preclinical models are needed to assess candidates at earlier stages. The pig is an ideal model system due to its human similarities in innervations, skin and the cardiovascular system. Other porcine models offered by MD Biosciences include post-operative pain, myocardial infarct, diabetes and diabetic neuropathy.

 

About MD Biosciences

MD Biosciences is a Preclinical and Clinical Contract Research Organization (CRO) providing services for biotech/pharmaceutical, medical device and animal health. Our core therapeutic focus is in inflammation/autoimmune, neurology/CNS disorders, pain and cardiovascular as well as the interplay between the inflammatory, neurology and cardiovascular systems. Our approach is to work backwards from the clinic, understanding what the clinicians would expect from an active compound. This approach and understanding enables the development of models and biomarkers that help break the preclinical/clinical barrier and provide more clinically relevant data at earlier stages.

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The information in this press release should be considered accurate only as of the date of the release. MDB has no intention of updating and specifically disclaims any duty to update the information in these press releases.

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Topics: Pain

Preclinical Imiquimod-induced Psoriasis-like Skin Inflammation Model

Posted by MD Biosciences on May 1, 2012 2:12:00 PM

A Rapid 10 day preclinical efficacy study that produces lesions highly representative of human psoriatic lesions.

Wednesday, May 2, 2012, St. Paul, MN – The most common models for evaluating skin-related inflammation are time consuming and expensive. While they provide valuable data, they don’t allow for rapid and cost effective evaluation of therapeutics earlier in the preclinical pipeline. In response to this need for more convenient and cost effective models, researchers (Van der Fits et al, 2009) developed a model using Imiquimod (IMQ) to induce psoriasis-like inflammation.

Imiquimod is a potent immune activator that is traditionally used to treat virus-associated skin abnormalities and precancerous skin lesions. However it can exacerbate psoriasis (both at the treated area and at distant skin sites) during topical treatment. Application of IMQ to mouse skin results in the influx of various cells of the immune system as well as hyperplasia of the epidermis that is critically dependent on IL-23 and IL-17.

MD Biosciences now offers the IMQ-induced model of skin inflammation as part of its portfolio of efficacy services. The model is 10 days long and the lesions closely resemble that of human psoriatic lesions with erythema, skin thickening and epidermal alterations such as acanthosis and parakeratosis. Subjects are given a psoriasis score depending on the degree of erythema, scaling and thickening. Analysis includes clinical score of the back, ear thickness and histology of both the ear and back.

 

Image: histology on psoriatic lesions (B-D) compared to that of a naive sample (A) in the IMQ-induced model of skin inflammation. For additional data, please visit the IMQ-induced model.

histology of the skin in the IMQ-induced psoriasis-like model of skin inflammation, preclinical CRO

 

About MD Biosciences

MD Biosciences is a Preclinical Contract Research Organization (CRO) providing services and products for biotech/pharmaceutical, medical device and animal health and companies engaged in inflammations & neurology research. With specialized laboratories located in Minnesota, Glasgow, and Israel, our panel of internationally recognized experts provides in-depth expertise and technologies to overcome challenges and provide total solutions to the drug discovery market.

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The information in this press release should be considered accurate only as of the date of the release. MDB has no intention of updating and specifically disclaims any duty to update the information in these press releases.

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Topics: Dermal