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MD Biosciences Blog

IHC Staining Image Gallery Now LIVE: High resolution whole slide image samples from in-house histopathology laboratory

Posted by MD Biosciences on Mar 21, 2019 11:57:33 AM

 MD Biosciences has extensive experience in the application of immunohistochemistry to analysis of markers of interest in tissue. Our validated protocols permit such assessments as lymphocyte analysis, cellular proliferation, cellular activation/differentiation, inflammation, biomarker analysis, and much more.

View high-resolution whole slide image examples of our IHC staining results below!

https://www.mdbhistopath.com/immunohistochemistry-image-gallery-ihc-markers 

 

Don't see a marker you're interested in? We provide custom IHC assay development and validation using either commercially available or Sponsor-provided antibodies, and additionally have in-house antibody development facilities for challenging targets. Contact us to discuss a custom project! 

Contact Us Today!  651-641-1770

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Topics: CRO/outsourcing, Biomarkers, Imaging, Pathology, GLP Histopathology, clinical research, Histology, GLP, In-situ Hybridization, Immunohistochemistry, Tissue Processing

De-Risking Human Clinical Studies: A New Pig Model for Nerve Block Therapies Assessment

Posted by MD Biosciences on Mar 18, 2019 11:42:36 AM

The problem: the rat model is limited in assessing any local treatment (cream, skin injections, patches and nerve blocks). 

The solution: translational models in pigs, prior to clinical stages.

  •  A cutting-edge translational model (technically and scientifically): volume of injection, nerve stimulation, imaging, PK, behavioral changes and skin biomarkers.
  •    By utilizing more translational models in larger species, the success rate of drugs entering clinical phases is dramatically increased.  

Now offering a unique model allowing the differentiation between motor dysfunction and pain block following drug application.

Screenshot 2019-03-13 11.53.29

Unique Model Characteristics

  • Similar to the clinic - ultrasound guide and nerve stimulation dosing
  • The study enables the differentiation between motor dysfunction and pain block following drug administration
  • Various readouts: von Frey-stimulation (primary pain), behavior score, motor function score, open field test, incision score, incision healing (histology) and biomarker analysis in skin

We also offer a wide variety of rodent models and services across therapeutic areas including inflammationpainneurodegenerative disease, wound healing, histologycell-based assays, biomarker analysis and statistical review and more to compliment your studies. We look forward to learning more about your research needs and working together. Call us today: 651-641-1770.

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Topics: neuropathic pain, translational research, porcine model

IHC Staining Image Gallery featuring CD3, T-cell co-receptor in tonsil tissue

Posted by MD Biosciences on Mar 15, 2019 12:20:36 PM

Below is a sample staining that demonstrates anti-CD3 IHC, 2x. CD3 is the T-cell co-receptor and is considered a Pan-T Cell lineage marker. T cells are seen surrounding B Cell Follicles in the below tonsillar tissue, demonstrating rich T-cell zones and interspersed t-cells within follicles. More high IHC quality images available upon request, and pathology review available in house.

Call us today for your histology needs: (651) - 451-1770.

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ValidationSlides_Tonsil_IHC_CD3_PanTCellMarker_2x-1

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Topics: Histology, GLP, Immunohistochemistry

Now Offering Synaptic Imaging Assay For Cognition and Neurodegenerative Disease Research

Posted by MD Biosciences on Mar 5, 2019 11:18:22 AM

 A powerful method for cognition, learning and degenerative disease research

 

MD Biosciences offers a synaptic imaging assay for evaluating the effect of different treatments on synapse formation and identification. This assay allows the estimation of inhibitory/excitatory synapses ratio and synaptic strength, a powerful method advancing cognition, learning and degenerative disease research and treatment options. 

Screenshot 2019-03-05 10.13.19

Unique Readouts:

  • Synaptic Count
  • Synaptic Strength
  • Identification of synapse type (excitatory/inhibitory)

 

Call or contact us today for more information!

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Topics: Neuro/CNS, assays, Neuroscience, Nerve Injury, cognition research

Senerga Cell-based Assay for Nerve Injury

Posted by MD Biosciences on Feb 19, 2019 11:26:36 AM

 A novel, rapid in-vitro assay for the screening of compound effects                                                              in nerve injury

Neurite outgrowth is the projection and extension of axonal processes from neuronal cell bodies. Through this process, neurons organize themselves into highly-complex functional networks connected synaptically to one another.


Modulation of this neurite outgrowth process (neuritogenesis) has been implicated in a wide range of CNS diseases, including neurodegenerative disorders such as spinal cord injuries, Parkinson’s Disease and Stroke.

 

MD Biosciences has developed a cell-based platform using primary cells extracted from adult rat, to evaluate the effect of tested compounds in the presence of inhibitory factors. The assay’s unique characteristics enable yielding more translatable results, which can serve as a predictive tool of the compounds’ efficacy in older animals and eventually in humans.

MD Biosciences offers an in-vitro assay to evaluate the effect of potential therapeutics on neurite outgrowth and connectivity (synaptic formation) in primary neuronal cell culture. 

image001


                                                                                     Neurite outgrowth after spinal cord injury in adult animal (Day 7)
Unique Screen Characteristics:

  1. Assays on spinal cord and cortex cell culture
  2. Primary neuronal cells extracted from adult animals
  3. Fast turnaround with results available in approximately 7 days
  4. Primary readout:  number of developed cells and the length of neurites in the presence of the tested compound and vehicle

Call or contact us today for our special introductory price of $1900 per compound. 

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Topics: Neuro/CNS, assays, Neuroscience, Nerve Injury

MD Biosciences GLP-approved Histopathology Laboratory launches new website: featuring histology, IHC, ISH and digital pathology services

Posted by MD Biosciences on Feb 15, 2019 9:00:00 AM

Our new histopathology website is now live! Check it out here!

MD Biosciences Laboratories offer a broad range of histology, immunohistochemistry (IHC) and In-situ hybridization services, staining services, tissue processing and digital pathology to enhance readouts and study applicability to the clinic.

We operate two distinct histology and IHC laboratories, including a GLP developmental research laboratory and a CLIA clinical IHC laboratory, offering a broad range of state-of-the-art technical capabilities to meet research and clinical study needs. Method development and optimization in addition to routine analysis shapes the foundation for our tissue-based services. The laboratories are equipped with advanced automated staining systems and digitized imaging, facilitating the privileged access to large, annotated image files. Services are complimented by the Visiopharm artificial intelligence-based image analysis platform to enhance the quality and speed of analysis. Services are supported by expert consultant pathologists specialized in various areas. 

Contact us to learn more about our services!

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Topics: CRO/outsourcing, Pathology, GLP Histopathology, Histology, GLP, Artificial Intelligence, In-situ Hybridization, CLIA certified, Immunohistochemistry, Tissue Processing

MD Biosciences Recognized As a Top 10 CRO by PharmaTech

Posted by MD Biosciences on Dec 13, 2018 11:42:24 AM

MD Biosciences was recently recognized by PharmaTech as a 'Top 10 most innovative CRO companies' for the European Market.

To learn more about recent technology advancements or have specific study inquiries, please contact us as we we would be happy to discuss with you. 

#biotechnology #innovation #CRO

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New MDB Publication: Pig open field data in the PNT model for neuropathic pain

Posted by MD Biosciences on Oct 12, 2018 11:00:00 AM

We recently published a publication demonstrating the utility of open field testing in combination with behavioral scoring in the pig peripheral neuritis trauma (PNT) model for pain. Combined together and applied in this model, they represent a powerful tool to assess the spontaneous behavior of pigs in response to pain. Rodent models are often used in pain research as they offer valuable data regarding underlying mechanisms contributing to pain, though they are limited in their translatability to human application. A pivotal benefit of using pig models is their increased translatability to the clinic considering the anatomical and physiological similarities pigs share with humans in comparison to rodents. This paper suggests that pig behavioral patterns are similarly translatable. MD Biosciences is proud to be a leader in neurology-related research, offering a variety of pig studies to enhance development in multiple therapeutic areas.
 

 For more information regarding our pig study services, visit our website or contact us!

 

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Topics: Pain, Stress, trauma pain, pain biomarkers, CNS imaging, Academic Researh, preclinical research, translational research, Behavioral Research, porcine model

Porcine incisional pain model pioneered by MDB scientists referenced in medical research journal

Posted by MD Biosciences on Jul 25, 2018 11:35:15 AM

MD Biosciences recognized in a paper published by Medtronic, a medical research company, to examine the efficacy of a sustained-release clonidine depot, placed in the wound bed, in a pig incisional pain model. To read the full paper, visit Dove Press Journal of Pain Research.

In addition to incisional pain models, other wound healing models offered at MD Biosciences are excisional woundings, heat wounds/burns, UV wounds and post-operative pain wounds. Contact us to discuss study-specific inquiries!

 

preclinical models of wound healing: full-thickness incisional and excisional 

 




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Pig post operative pain model pioneered by our scientists recognized in a scientific publication: Synergistic Effect of Bupivacaine and Meloxicam in Clinical & Animal Studies

Posted by MD Biosciences on Jul 12, 2018 11:45:29 AM

Proud to announce that the pig post operative pain model pioneered by our scientists was used and cited in a clinical study poster publication written by Heron Therapeutics to assess the synergistic effect of Bupivacaine and Meloxicam in HTX-011.

"Meloxicam and Bupivacaine combined in a single extended-release formulation (HTX-011) delivered at the wound site in a preclinical post-surgical pain model in pigs exhibited greater analgesia than either compound delivered alone within the same extended-release formulation; this finding was confirmed in an initial clinical trial in bunionectomy" (Heron Therapeutics, 2018).

To learn more about our rodent and pig post-operative pain models, visit our preclinical pain webpage or view  our datasheet below.

pig post-operative pain (POP) model, preclinical efficacy 

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Topics: Pain, post-operative pain, neuropathic pain, preclinical research