In the April 2009 issue
A new animal model for post-operative pain
Data comparing IV and IP administration in the CAIA model
Recent Peer Publications
Measuring OVA-specific IgE levels
Human Type I Collagen ELISA
A new animal model for post-operative pain. Monitor wound healing and pain simulateously.
The under treatment of post operative pain has been recognized to delay patient recovery and discharge from the hospital. Despite recognition of the imporantance of effective pain control, up to 70% of patients still complain of moderate to severe pain post-operatively. The most commonly used model to test the effect of new analgesic drugs in post operative pain is the Brennan model in rats. Although this model can provide good answers to systemic drugs, it is less suitable for testing local drugs such as implants, patches, medical devices and creams.
MD Biosciences has developed a unique post-operative pain model in pigs to meet this growing need. The model incorporates the assessment of post-operative pain and the observation of wound healing for up to 16 days post-surgery, making it an ideal model for the assessment of local treatments.
Download the whitepaper describing the New Animal Model of Post-Operative Pain.
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New data comparing IP vs IV administration of ArthritoMab™ Antibody Cocktail.
The Collagen Antibody Induced Arthritis (CAIA) model is a rapid and synchronized model that offers researchers an alternative to the lengthy CIA model for the study of Rheumatoid Arthritis (RA). The model can be completed in as little as 10 days with histological findings of cellular infiltration, synovial hyperplasia and bone/cartilage erosion.
The standard protocl for administering the antibody cocktail is via intravenous injection into the tail vein. For labs that are unable to administration IV due to ethical restrictions or labs that simply don't prefer this route, administration via IP injection may be used. While it has been previously claimed that administering antibody cocktail via IP will reduce the severity of arthritis, the period of active inflammatory arthritis is shorter and it will require more antibody cocktail, studies using MD Biosciences ArthritoMab™ antibody cocktail indicate that administration via IP performs as equally well as iv (figure 1). In fact, total scores of both front and rear paws indicate that tissue distribution of antibody after IP administration may be better than IV, leading to higher inflammation in the fore paw (figure 2).
For further information on the ArthritoMab antibody cocktail and its use in the Collagen Antibody Induced Arthritis Model (CAIA), download the whitepaper.
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Recent Peer Publications
Adaptor Proteins and Ras Synergystically regulate IL-1-Induced ADAMTS-4 Expression in Human Chondrocytes 1
Rasheed Ahmad et al., (April 2009) J Immunol 182:5081
Product: Sensitive Aggrecanase Assay (Catalog # M046009/SEN-AGG.96)
Application: Quantitative determination of aggrecanase activity in cell culture supernates.
Mast cell chymase contributes to the antibody response and the severity of autoimmune arthritis.
Magnusson, S et al., (March 2009) Faseb Journal 23:876
Product: ArthritoMab™ Antibody Cocktail (CIA-MAB-50)
Application: Induction of antibody-mediated arthritis
Lymph Node IL-18 expression in adult-onset Still's Disease
P Conigliaro et al., (March 2009) Ann Rheum Dis 68:442
Product: IL-18 Monoclonal Antibody (Catalog 201006)
Application: Immunohistochemistry
Type XIV Collagen Regulates Fibrillogenesis. Premature Collagen Fibril Growth and Tissue Dysfunction in null mice.
Ansorge, H et al., (March 2009) J Biol Chem 284(13):8427
Product: Antibody against Type I Collagen (Catalog MD20151)
Application: Indirect immunofluorescent staining
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Measuring OVA-specific IgE Levels in a mouse model of allergic asthma.
Allergic Asthma is typically triggered by allergans in the air such as pollen, mold, dust mites etc. It can be characterized by reversible airway obstruction, elevated levels of IgE causing mast cell activation, chronic airway inflammaton and airway hyperresponsiveness. The immunological processes involved are characterized by proliferation and activation of Th2 lymphocytes. The Th2 mediated allergic inflammation is accomplished with cytokines such as IL-4 and IL-13 for their role in directing b lymphocytes to synthesize IgE, IL-5 for its role in maturation and activation of eosinophils, IL-13 and IL-10 for their role in mast cell genesis as well as GM-CSF.
While we know that Th2 lymphocytes play an important role in the initiation, progression and persistence of allegic asthma, there is much to be understood about the immunoregulatory mechanisms. A widely used model for studying allergic asthma and proposed therapies is the OVA-induced Asthma model in mice. Following sensitization and challenge with OVA, there is a significant increase in the number of inflammatory cells in the bronchoalveolar lavage fluid. Eosinophils predominate the granulocyte response with an increase in T lymphocytes. The asthma induction process also results in significantly more anti-OVA IgE in the serum of mice, which can be quantitated using the mouse OVA-IgE ELISA.
Research Tool for the study of Allergic Asthma
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Human Collagen Type I ELISA for the measurement of collagen I production in human cells/tissue.
Collagen Type I is the most abundant protein and is found in the skin, connective tissue, tendons, ligaments, cornea, intevertebral disks and bone. It has fund to be involved in many human diseases such as fibrosis, osteoporosis, cancer and artherosclerosis. The human Collagen Type I ELISA provides an easy to use assay to detect and quantify type I collagen in cell culture supernates.
Benefits:
- Pre-coated, ready to us microplate: eliminates overnight plate coating step found with other commercially available kits.
- Rapid - you can have results in under 3 hours
- Easy to use: all reagents are included for quick and easy set up
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