In the March 2009 issue
Antibodies to Citrullinated Protein
Antibodies to structural components of ECM
IHC staining of articular cartilage
Detecting SLRP core protein fragmentation in meniscus, knee and hip articular cartilage.
New antibodies to Citrullinated Protein.
Citrullination of proteins is a post-translational modification where arginine is converted into citrulline by a family of enzymes called peptidylarginine deaminases (PADs). Proteins are susceptible to citrullination during inflammatory processes, and an autoantibody response to citrullinated proteins is a hallmark of Rheumatoid Arthritis (RA).
| Antibody |
Type |
Cat # |
| anti-Citrullinated CII, clone ACC-1 |
Monoclonal |
1041003 |
| anti-Citrullinated CII, clone ACC-4 |
Monoclonal |
1041004 |
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Antibodies to Structural components of the ECM (proteoglycans and glycosaminogycans)
Immunohistochemical composition of neocartilaginous graft ECM. Sections have been labled (green) with antibodies toward structural components of the extracellular matrix, i.e. glycoaminoglycans and proteoglycans. Cell nuclei have been counterstained red with propidium iodide.
(full data referenced: Anthony Hayes, Amanda Hall, Liesbeth Brown, Ross Tubo and Bruce Caterson (2007) J Histochemistry Cytochemistry 55(8):853.)
Antibodies from data above:
| Antibody |
Clone |
Treatment |
| Antibody to C-S-4 and DS neoepitope |
2B6 |
ABC/K (C-4-S)
B/K (DS) |
| Antibody to Keratan Sulphate |
5D4 |
none |
| Antibody to Native Bovine Lubricin |
3A4 |
none |
| Antibody to Aggrecan IGD |
6B4 |
ABC/K |
ABC- chondroitinase ABC, B-chondtroitinase B, K- keratanase
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BC-3 and BC-13 monoclonal Antibodies: IHC staining of articular cartilage.
Immunohistochemical staining of human articular cartilage from a patient with osteoarthritis of the knee using monoclonal antibodies BC-3 and BC-14.
Osteoarthritis (OA) is a chronic and debilitating joint disease that is characterized by progressive destruction of cartilage wherease Aggrecan degradation is initially observed followed by overt collagen degradation.
In the figure below, sections from osteoarthitic human articular cartilage were either stained with (a) Toluidine Blue (b) monoclonal antibodies BC-4; anti MMP-generated neoepitope...DIPEN or (c) BC-13, anti-aggrecanase generated neoepitope ....NTEGE. Toluidine blue staining indicates loss of proteoglycan staining (blueness) in the fibrillated surface zone of the cartilage. Immunostaining with BC-4 shows staining in the mid-zones of the articular cartilage in the territorial matrix. In contrast, immunostaining with mAb BC-13 shows staining in the surface zone and uppoer mid-zone.
Aggrecan Antibodies:
| Antibody |
Cleaved by |
Application |
Aggrecan antibody to N-terminal
neoepitope ARG (clone BC3) |
Aggrecanase |
WB, ELISA, IHC |
Aggrecan antibody to N-terminal
neopeitope DIPEN (clone BC4) |
MMPs |
WB, ELISA, IHC |
Aggrecan antibody to N-terminal
neoepitope NITEGE (clone BC13) |
MMPs |
WB, ELISA, IHC |
Aggrecan antibody to N-terminal
neoepitope FFGV (clone BC14) |
Aggrecanase |
WB, ELISA, IHC |
| anti-IGD Aggrecan antibody (clone 6B4) |
- |
WB. ELISA, IHC |
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Detecting Small Leucine Rich Proteoglycan (SLRP) Core Protein Fragmentation in meniscus, knee and hip articular cartilage.
Using SLRP Antibodies for Western Blotting of tissue extracts.
Data from Melrose et al., (2008) Arthritis Research & Therapy 10:R79
Presented is identification of intact SLRP proteins and fragments in age-matched macroscopically normal (N), osteoarthritic (OA) or fibrillated (F) knee articular cartilage (AC). Affinity-purified anti-caroxyl-terminal SLRP antibodies (PR-84), PR-85, PR-184) and PR-353) and a monoclonal antibody to full-length keratocan core protein (KER-1) to perform western blots of samples. The brackets above the lanes indicate that macroscopically normal and fibrillated articular cartilage were samples from the same individual monoarthritic joint for comparison.
Identification of SLRP core protein fragmentation in meniscus, knee and hip articular cartilage.
Presented is indentification of SLRP core protein fragmentation in 4M GuHCl extracts of meniscus, knee and hip articular cartilage of individual tissue specimens from total knee or hip replacement patients. The ages (years) of each specimen art indicated at the top of each lane.
| Antibody |
| Monoclonal antibody to Keratocan |
| Monoclonal antibody to Lumican |
| Aggrecan antibody to N-terminal neoepitope ARG, clone BC3 |
| Aggrecan antibody to N-terminal neoepitope DIPEN, clone BC4 |
| Aggrecan antibody to N-terminal neoepitope NITEGE, clone BC13 |
| Aggrecan antibofy to N-terminal neoepitope FFGV, clone BC14 |
| anti-IGD Aggrecan antibody, clone 6B4 |
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