Inflammatory Assays

Mouse OVA IgE ELISA

Mouse OVA IgE ELISA				Catalog # OVA-IGE96
Format	Sandwich ELISA				View product insert
Size/Volume	96 well
Species	mouse				Request Quote
Sample Type	serum, cell culture supernate
Sample Preparation	dilute samples at least 1:10
Sample Size	100 uL
Standard Curve Range	7.8 - 500 ng/mL
Sensitivity	< 3.78 ng/mL
Assay Length	2.25 hours

 

OVA IgE OVERVIEW

IgE is the least abundant isotype but has the capability of triggering powerful immune responses by binding to Fc receptors on the surface of cells such as mast cells, basophils, eosinophils, monocytes, macrophages and platelets.

IgE is well known for its involvement in eliciting an allergic or asthmatic response.  Ovalbumin (OVA) is widely used for inducing an allergic response in mouse models of allergic asthma. Through T and B cell signalling, IgE production is stimulated and released into the bloodstream where it attaches to receptors on cells such as mast cells and basophils. These cells are then activated upon cross-linking of the IgE with the antigen initiating the allergic cascade.

Intended use

The Mouse OVA IgE ELISA provides a method for the determination of OVA specific IgE in mouse serum and cell culture supernate samples.

Principle of the test

ELISA or enzyme-linked immunosorbent assay is a colorimetric based immunoassay utilizing a capture antibody and a detection antibody to provide a unique and powerful assay system. Antibody/antigen reactions take place on the surface of microplate wells that have been previously coated with monoclonal antibody to OVA IgE. Biotinylated ovalbumin and strept-avidin peroxidase, in the presence of substrate quantifies the analyte bound.

 

Product inserts are for information use only.