T cell activation assay: preclinical research

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Stable Luciferase Reporter In Vitro Assay: Contract Research

 

Stable cell lines containing a luciferase reporter gene driven by transcription factor response elements are used to screen for compounds that activate or inhibit a particular cell signaling pathway. Please inquire if your reporter of interest is not included below.

 

Stable Luciferase Reporter In Vitro Assay    Get Proposal
Cell System STAT-1 Reporter HeLa Stable Cell Line
NF-kB Reporter HeLa Stable Cell Line
CREB Reporter 293 Stable Cell Line
AP-1Reporter 293 Stable Cell Line
Length 1 week
Stimulant STAT-1: INF-g
NF-kB: TNF-a
CREB: Forskolin
AP-1: PMA
Reference Inquire
Readouts

luciferase activity

 

stable luciferase reporter assays for screening compounds that activate or inhibit cell signaling pathways. preclinical Contract research

Figure: Transcription factor reporter strain assay. Cells harboring a luciferase reporter gene driven by STAT-1, NF-kB, CREB or AP-1 response elements were treated with the appropriate stimulant. Luciferase activity, a measure of transcription factor pathway activation, was determined. Mean luciferase fold-induction values are shown. Error bars represent standard deviations. 

 

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