House Dust Mite-Induced Asthma

HDM Extract-Induced Allergic Asthma Model

The House Dust Mite (HDM) murine model of allergic asthma is used to assess therapies with potential indications in airway inflammation and/or pulmonary inflammation driven primarily by repeated exposure to allergens. As roughly 85% of allergic asthma sufferers have increased HDM-specific serum IgE levels, the HDM model demonstrates significant relevance for investigating the molecular mechanisms of allergic asthma pathogenesis.

HDM-induced asthma recapitulates many of the features of human allergic asthma, including a primarily Th2- associated cytokine response, eosinophilic infiltration into the lungs, airway hyper responsiveness (AHR) and epithelial tissue remodeling. The model consists of a subcutaneous HDM sensitization followed by intranasal challenge of HDM. Main readouts include broncheoalveolar lavage fluid (BALF) cellularity assessment and cytokine production, with optional blood and lung tissue collection for further downstream analysis.

Assessments:

• Cell populations in the BALF by FACS
• Cytokine profile in the BALF or lung tissue homogenate by multiplex
• IgE levels
• Total Protein
• Histology

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