TNBS-induced IBD & Gastrointestinal Inflammation Models

TNBS-induced intestinal inflammation is commonly used in mice as a model for IBD, particularly in reference to Crohns disease (CD).

IBD Model Utilizes Intra-rectal Administration of TNBS

For our 7-day TNBS model, we induce disease through intrarectal administration of TNBS, to produce clinically amenable indicators to that of Crohn's Disease. Indicators include: diarrhea, rectal bleeding, body weight loss, more diffuse intestinal inflammation, occasional adhesions, fibrosis and vascularized ulceration. Disease indications can also be significantly reduced for a less servere phenotype via a one-time i.p. injection of anti-p40 antibody.

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Dosing Routes:	Assessments available:
PO	Body weight loss
IV	Colitis score
IP	Colon length/weight
SC	Gross Pathology
Infusion Pump	Biomarkers
	Histology/IHC
	PK

Colonic Sections from TNBS-Induced IBD Model in Mice Processed via "Swiss Roll" Technique to Preserve Morphology (Periodic Acid Schiff/PAS Staining):

TNBS-Model-IBD-Periodic-Acid-Schiff-Staining-Colitis-Mucins-Polysaccharides.png

Figure A. Medium magnification of PAS-stained colon sections demonstrating mucins and polysaccharides. Figure B. High magnification of PAS-stained section in A.

Colitis Scores in the TNBS Model

Balb/c were administered TNBS intra-rectally. Over the course of 7 days, colitis score was monitored in animals receiving vehicle and positive controls anti-IL-12 p40.

Preclinical Laboratory Services

TNBS-Induced Model of IBD

Colitis Scores in the TNBS Model

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