Nerve blocking agents are often used to replace general anesthesia in certain surgical procedures or for providing pain relieve in the post-operative period where the nerve block tends to provide more effect pain control and reduce opioid-related side effects. The rapid preclinical model of sciatic nerve block enables researchers to directly access and dose to the sciatic nerve and allows the screening of new nerve blocking agents or drugs designed to reverse the local analgesia. The nerve block model is based on the administration of nerve blocking agents directly to the saphenous and sciatic nerves. Local injection is performed to the adductor canal as well as to the sciatic notch. Following the administration of nerve blocking agents, thermal hyperalgesia is tested for a duration relevant to the nerve blocking agent. The capability can be applied to other models such as the SNL and CCI models. The ability to access the sciatic nerve directly opens up options for drug developers. For instance, those who are working with ion channel blockers now have the ability to dose directly to the sciatic nerve rather than systemic administration.
Paw sensitivity is measured on study Day -1 (baseline) and then on study Day 0 post TI’s injection at time points 5, 10, 15, 20, 60 and 120 minutes. Latency to move the foot is recorded in seconds. The intensity of the light will be adjusted with latency of normal paw at approxi- mately 12 seconds and a cut-off latency of 30 seconds.