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Pain-related characteristics in MIA-induced preclinical model of OA

  
  
  

Osteoarthritis (OA) is a widespread condition that affects greater than 70% of the elderly population and poses a heavy cost burden on healthcare. It is a chronic degenerative disease characterized byt the loss of articular cartilage components, which affects the entire joint structure. One of the major complaints by OA patients is the loss of joint function as well as chronic pain. Current therapies are focused on alleviating joint pain, however full pain relief is rarely experienced and significant side affects are commonly present. Research is not only focused disease pathology but also on understanding the mechanisms responsible for induction and maintenance of pain states.

The monosodium iodoacetate (MIA) model has been well characterized for the evaluation of OA pain. The iodacetate creates local inflammation in the knee from fluid expansion of the synovial mebrane, which is then followed by the production of inflammatory mediators (TNF-a, IL-1b, IL-6 and NGF). These mediators further contribute to OA pathogenesis by increasing cartilage degradation. The cartilage damage and degradation leads to chronic neuronal damage with neuropathic characteristics. The MIA-OA model then is thought to contain both inflammatory and neuropathic pain-related states by using local tissues and sensory innervation of the peripheral nervous system.

Inflammatory-related Pain State

CGRP (calcitonin gene-related peptide) is elevated in the sensory neurons innervating the knee and DRG (dorsal root ganglia). CGRP is an inflammatory driven neuronal marker whose expression is dependant on NGF (nerve growth factor) expression. The elevation of CGRP produces hyperalgesia and a pain sensation derived from inflammation.

Neuropathic-related Pain State

It is also suggested that proliferation of microglia in the dorsal horn of the spinal cord along with elevated levels of pro-inflammatory cytokines contributes to neuronal damage. The neuronal damage gradually rises along with the progress cartilage damage and this may lead to the chronic neuropathic pain state. 

MIA-induced OA model

The MIA-induced OA model can allow the evaluation of anti-inflammatories (to treat early symptoms) as well as analgesics (to treat chronic pain). The study can be run anywhere from 14 days out to 56 days or longer depending on the proposed therapy. 

Readouts: 

  • Weight bearing measurements: weight bearing measures the spontaneous pain as a result of nerve injury or inflammation. 
  • Open field: the open field test would measure the animals desire to walk, the distance and speed if they did walk. 
  • Rotorod: the rotorod test would measure the ability to withstand the pain in a situation where walking was required. 
  • Joint compression: Randall Selitto can be used to measure the pressure required to withdraw (compression threshold)
  • Histology and xray analysis of the knee
  • Biomarkers (mRNA and protein level)
MIA-induced osteoarthritis, weight bearing, inflammatory pain, neuropathic pain, preclinical CRO
Weight bearing measurements (% of weight carried on ipsilateral leg) for gabapentin and vehicle groups in the MIA-induced OA model.
MIA-induced OA model, preclinical pain contract research, pre-clinical CRO
Weight bearing (% difference between left and right leg) of gapabentin and vehicle control in the MIA-induced model of OA.
References
  1. Orita, et al. (2011) Pain-related sensory inervation in monoiodoacetate-induced osteoarthritis in rat knees that gradually develops neuronal injury in addition to inflammatory pain. BMC Musculoskeletal Disorders 12:134
  2. Harvey, V and A. Dickenson (2009) Behavioral and electrophysiological characterisation of experiementally induced osteoarthritis and neuropathy in C57BL/6 mice. Molecular Pain 5:18
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