Preclinical Pharmacology & Efficacy

Chronic Constriction Injury

Peripheral Nerve Injury (PNI) Models of Neuropathic Pain

Chronic Constriction Injury (CCI) Model of Neuropathic Pain.

In a typical CCI model, the left sciatic nerve is ligated causing inflammation and swelling, and ultimatly peripheral pain. This method results in a severe and disproportional reduction of large myelinated fibers. Although significant disruption of small unmyelinated C-fibers occurs as well, many remain intact. This allows ongoing pain behaviors and increased sensitivity to mechanical stimuli to be observed following surgery. 

Enhanced CCI Model

In our standard model, the left sciatic nerve is exposed in anesthetized animals above the femoral joint. One loose slik suture knot and 3 cat gut sutures are applied to the sciatic nerve. The skin is then clamped closed. One week later, the animals pain threshold is assessed using the Von Frey filament test. Animals that demonstrate reduced threshold in the operated leg are included in the study and divided into different treatment groups. Their pain threshold is then re-measured following compound dosing.

Model Specifications

  • Model length - Up to 30 days
  • Positive controls: Morphine, gabapentin, pregabalin
  • Readouts: Tactile allodynia (Von Frey)
  • Endpoints: Histology, biomarker analysis (protein or mRNA) in sciatic nerve or serum/plasma

 

Don't see the model or assessment you require? Our scientists are experienced with rapidly validating models from the literature. Please speak with a scientist to discuss your requirements. 

 

SPECIES AVAILABLE:

  • Rat (Sprague Dawley)
  • Mouse (C57/Bl6)

 

 

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clinically relevant model of neuropathic pain

 

 


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