Peripheral Nerve Injury (PNI) Models of Neuropathic Pain
In a typical CCI model, the left sciatic nerve is ligated causing inflammation and swelling, and ultimately peripheral pain. This method results in a severe and disproportional reduction of large myelinated fibers. Although significant disruption of small un-myelinated C-fibers occurs as well, many remain intact. This allows ongoing pain behaviors and increased sensitivity to mechanical stimuli to be observed following surgery.
Enhanced CCI Model
In our standard model, the left sciatic nerve is exposed in anesthetized animals above the femoral joint. One loose silk suture knot and 3 cat gut sutures are applied to the sciatic nerve. The skin is then clamped closed. One week later, the animals pain threshold is assessed using the Von Frey filament test. Animals that demonstrate reduced threshold in the operated leg are included in the study and divided into different treatment groups. Their pain threshold is then re-measured following compound dosing.
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Von Frey (tactile Allodynia) measurements in the rat CCI model of peripheral nerve injury