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MD Biosciences Blog

Qualitative and Semi-Quantitative Analysis of RNA Expression

Posted by MD Biosciences on Oct 20, 2020 11:30:00 AM

In-Situ Hybridization (ISH) and Immunohistochemistry (IHC) Services

Understanding biomarker expression patterns visually within tissue is crucial to our understanding overall physiology, allowing us to better perceive and diagnose diseased states. Both immunohistochemistry (IHC) and RNA in situ Hybridization (ISH) provide images allowing for assessment of localization and quantification of marker expression, while preserving the tissue integrity. IHC and ISH can be used together to validate data or provide complementary information. 

 

The combination of both technologies can provide unique information. It can identify a secreted protein (like growth factors or cytokines) and its origin, identifying both the original cell and protein within the tissue sample. ISH can also be used for detection and quantification of the RNA target inside the tissue. The simultaneous analysis of RNA and protein expression of the same target genes allows for the identification of potential gene expression regulation mechanisms.

 

MD Biosciences offers fully automated IHC and ISH testing, which can be performed using both brightfield and immunofluorescence staining as singleplex or multiplex assays. In addition, we have the capability to combine IHC/ISH testing, allowing for protein/gene expression comparison simultaneously. With whole slide imaging and hosting capabilities, remote viewing, annotation and downloads are easily accessible. Moreover, we offer pathologist review and/or quantitative AI-powered image analysis.

Additional IHC Services


How can our histology team assist with your research? Contact a scientist to discuss customized options that fit your needs! 

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Topics: Biomarkers, Pathology, ID Markers, clinical research, clinical diagnostics, Biotechnology, Histology, GLP, Artificial Intelligence

Evidence Mounts for the Role of Inflammation and Immune Dysregulation in Metabolic Liver Disease Related to Obesity

Posted by MD Biosciences on Jul 19, 2018 9:30:00 AM

Over the past couple of decades, nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) have become the number one cause of liver disease in Western countries. Recent data confirm that NAFLD and NASH play an equally important role in the Middle East, Far East, Africa, the Caribbean, and Latin America. The prevalence of NAFLD/NASH continues to increase along with its associated risk factors, including metabolic disorder, obesity, diabetes and hypertension. Therefore, understanding the mechanism of these diseases has become a priority. Recent investigations into the effect of chronic liver inflammation have shed light on the role of both the adaptive and innate immune systems in both NAFLD and NASH. Long suspected to play a part in such metabolic disorders along with nutrition and hormonal imbalances, the immune system has been of great interest to researchers for development of specific drug targets to maximize glucose regulation, minimize inflammatory damage to tissues and prevent the development of fibrosis that can lead to liver failure.

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Topics: Inflammation, metabolic, Pathology, murine model, preclinical research, fibrosis

Biomarkers Put Precision In Precision Medicine | AMP 2015

Posted by MD Biosciences on Nov 12, 2015 12:58:51 PM

The 2015 annual AMP meeting opened Novemeber 5th in Austin TX, with a presentation by Dr Brian Druker titled “Imatinib as a Paradigm of Targeted Cancer Therapies.”  This year’s recipient of the AMP Award for Excellence in Molecular Diagnostics, Dr Druker highlighted the importance of identifying patients through biomarkers and matching therapies to the patient.  This theme continued through many of the presentations, posters and vendor exhibits the importance of providing physicians actionable results for patient care and therapeutics.  Molecular diagnostics are critical to any discussion on precision or personalized medicine. 

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Topics: cancer/tumor, Microbiome, Biomarkers, Association for Molecular Pathology, Pathology, precision medicine

Join MD Biosciences for AMP 2015 Annual Meeting

Posted by MD Biosciences on Nov 2, 2015 2:30:00 PM

MD Biosciences will be attending the Association for Molecular Pathology 2015 Conference in Austen Texas on November 5-7. The conference will focus on “realizing the dream of precision medicine”. As a CLIA lab, we are looking forward to meeting with our colleagues in the diagnostic industry and discussing the latest updates in testing and treatments in cancer and infectious diseases. Additionally, we are excited to hear about the cutting edge research occurring in clinical translational medicine and pharmacogenomics. Please contact us at info-us@mdbiosciences.com if you would like to meet at the conference. Stay tuned for our blog recap of the event.

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Topics: AMP, Association for Molecular Pathology, Pathology, CLIA


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